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pi bu zhi fang zu zhi nei fen mi huo dong yu jing luo xian xiang xiang guan xing de yan jiu tu qian zhi fang xi bao de yuan dai pei yang
Author(s): 
Pages: 46-48
Year: Issue:  S1
Journal: Chinese Acupuncture & Moxibustion

Keyword:  前脂肪细胞 原代培养 脂肪组织;
Abstract: 目的:原代培养家兔前脂肪细胞并诱导其分化为成熟脂肪细胞,为针灸血清经络实验建立活细胞模型。方法:选用兔腹股沟处皮下脂肪组织,采用原代消化法培养,通过对培养细胞进行形态学观察及用油红O脂肪染色法进行染色以鉴定。结果:形态学观察及油红O脂肪染色法测定证明是脂肪细胞,针灸细胞分子机制和经络现象研究的活细胞模型成功。结论:该实验建立的体外培养兔脂肪细胞的结果,可为研究脂肪组织内分泌活动与经络现象的相关性提供模型基础。
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