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Cloning and Analysis of ACTB Gene of Guizhou Xiang Pig
Author(s): 
Pages: 7-10
Year: Issue:  2
Journal: Feed Review

Keyword:  Guizhou xiang pigACTB genegene cloningsequence analysis;
Abstract: 以贵州香猪肌动蛋白基因的RNA序列为模板,运用PCR技术扩增香猪ACTB基因的CDS区,将其插入PMD19-T克载体中,导入工程菌E.coliDH5a感受态细胞中,并进行相关的分析。结果表明,PCR产物长度为1596bp,编码一个由376个氨基酸组成的多肽,与GeneBank上发表的序列大小相符。
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