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Standardization Fluorescence Quantitative Polymerase Chain Reaction Process
Author(s): 
Pages: 13-17
Year: Issue:  4
Journal: Feed Review

Keyword:  RT-qPCRMIQERNA extractionRT-PCRprimer designdata analysis;
Abstract: 实时荧光定量PCR(RT-9PCR)是把酶动力学、核酸扩增与杂交、光谱分析和实时检测技术巧妙结合的一项技术,具有特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等特点,因此广泛应用于病毒学、遗传性疾病、肿瘤和癌症的诊断等方面,成为分子生物学研究中的重要工具。文章根据MIQE指南简要介绍RT-qPCR规范的试验流程。
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