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chan huang qing mei jun (penicillium chrysogenum) de bing du ji qi shuang lian he tang he suan de jian ding
Author(s): 
Pages: 21-27+91
Year: Issue:  1
Journal: Acta Microbiologica Sinica

Keyword:  病毒核酸双链核糖核酸产黄青霉菌病毒颗粒上清液显色反应柠檬酸电子显微镜检查青霉素产生菌毫微米;
Abstract: 用电子显微镜检查了4个青霉素产生菌产黄青霉(Penicillium chrysogenum)菌株,在其中3个P1001、6205和r3,观察到球形病毒颗粒。5562在平面培养中产生"蚀斑",但未看到病毒颗粒。菌丝匀浆经15000—23000g离心的上清液加柠檬酸调到pH 4.8,可使病毒沉淀,简化了提取方法。病毒在pH 7.0 0.02M磷酸钠缓冲液,4℃中保存20天后大部分破坏,而保存在-5℃经一次冻融则影响较小。病毒核酸白色线伏,二苯胺显色反应阴性,苔黑酚显色反应阳性,2.4%聚丙烯酰胺凝胶电泳出现3条带,经热变性、对核糖核酸酶抵抗性和甲醛作用的实验证明是双链核糖核酸。
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