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yi zhong ling min jian ce zhi wu bing du de fang she mian yi xi fu ce ding fa
Author(s): 
Pages: 46-51
Year: Issue:  4
Journal: he nong xue tong bao

Keyword:  植物病毒球蛋白放射免疫吸附测定花椰菜花叶病毒分离株酶结合物免疫学提纯酶联免疫吸附法双抗体夹心;
Abstract: <正> 引言业已证明,检测植物病毒时,酶联免疫吸附法(ELISA)比免疫扩散、沉淀素以及其它常规血清学方法灵敏得多。ELISA法通常用于检测浓度为10—100ng/ml的病毒。虽然对某些病毒来说最低检测浓度可低至1ng/ml以下,对另一些病毒可高达500ng/ml。我们在试图应用ELISA法在含有少量感染种籽的莴苣样品中(500—1000粒中含有1粒感染种籽)
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