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wei fu jiao gan nei zang yun dong jie qian xian wei de fa duan hrp fa yan jiu
Author(s): 
Pages: 182-189
Year: Issue:  3
Journal: Acupuncture Research

Keyword:  神经运动标记细胞背核副交感迷走神经节染色质溶解纤维节前胃壁内脏;
Abstract: Up to now, the problem of the origin of the parasympathetic pregan- glia fibers of stomach is still at issue. Somebody considered that the pre- ganglia fibers of visceral motor of vagal nerve originated in the nodal ganglion; some considered that the dorsal motor nuclei of vagus did not innervate the visceral smooth muscle, and some suggested that stomach was innervated doubly by the dorsal motor nuclei of vagus and the medialis nuclei of tractus solitarii. Thus, We think that it is necessary to further study the origin of the parasympathetic preganglia fibers of the stomach. 24 rabbits were used in this study. 80-100ul of 10% HRP, dissolved in 0.9% saline, were injected into the tunica muscularis of the stomach of rabbits. Among them, 6 were injected in the anterior wall of stomach. 6 in the posterior wall, 6 in the cardiac pars and 6 in the pyloric pars. After a survival period of 5 days the animals were intracardially perfused with 2% paraformaldehyde in 0.1M phosphate buffer (PH7.2-7.4). The medulla were removed and cut serially at 40μm on a freezing microtome. The sections were divided into two parts and treated with 3', 3, diamino- benziue (D.A.B) and benzindine hydrochloride (B.D.H.C) separately. Sections were conterstained with neutral red. The HRP labelled cells were found within the dorsal motor nuclei of vagus in the present study. The projective area of stomach is situated in the supra-middle part of these nucle, close to medialis, the marked level of obex from above the obex 2-3 mm to below the obex 1-2mm, totally about 4-5mm cephalocaudally. most of these cells, nearly 93% of the total labelled cells, are small ones. The four different parts of the stomach, anterior wall, posterior wall, cardiac and pyloric pars, do not occupy specific location within the dorsal motor nuclei of vagus. In the present study the HRP labelled cells were only found within the dorsal motor nuclei of vagus, but the HRP labelled cells were not seen in the medialis nuclei of tractus solitarii. This study shows that the dorsal motor nuclei of vagus join to adjust the functional activity of the stomach Our previous study showed the connection of the dorsal motor nuclei of vagus with the sensory fibers of trigeminal nerve, Therefore, the inhibition of the stretch reaction of stomach is completed by the dorsal motor nuclei of vagus when the facial point are accurpunctured.
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