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yan suan du luo xi ting de han liang ji qi you guan wu zhi de rp-hplc ce ding fa
Author(s): 
Pages: 1223-1225
Year: Issue:  10
Journal: Occupation and Health

Keyword:  RP-HPLCDuloxetine HydrochlorideRelated substances;
Abstract: 目的建立反相高效液相色谱(RP-HPLC)法测定盐酸度洛西汀含量及其有关物质。方法色谱柱为美国Diamonsil(250 mm×4.6 mm,5μm)C18反相柱;流动相为乙腈-甲醇-缓冲液=30∶30∶40,流速为1.2 ml/min;检测波长230 nm。结果盐酸度洛西汀浓度在4.8~48.0μg/ml范围内,线性关系良好,标准曲线为A=5 147.5C+4.710 7(r=0.999 8,n=7),定量下限0.5μg/ml。结论 RP-HPLC法具有快速、简便、灵敏度高、重现性好等优点,可以用于盐酸度洛西汀的含量测定及有关物质检查。
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