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Determination of IMP in poultry muscle by HPLC
Author(s): 
Pages: 667-670
Year: Issue:  5
Journal: Journal of Southwest University for Nationalities(Natrual Science Edition)

Keyword:  家禽肌肉肌苷酸高效液相色谱法;
Abstract: 目的:建立HPLC法测定家禽肌肉中IMP含量的方法.方法:采用D1KMATM Platisil(铂金)ODS C18柱(150×4.6mm),流动相A∶0.04M磷酸二氢钾0.06M磷酸氢二钾用0.5M和5M调至PH为7流动相B:甲醇,柱温:25℃,检测波长为254nm,流速1mL/min.结果:IMP在5μg/ml~250μg/ml范围内呈良好的线性范围,回归方程为y=0.0843x-1.5187,R2=0.9994.结论:该法简便可靠,能准确测定家禽肌肉中IMP的含量,适用于肉品质及其风味物质的准确性的检测.
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