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Development of A PCR Method for Rapid Detection of Bacillus Licheniformis Based on gyrB Gene
Author(s): 
Pages: 29-31
Year: Issue:  3
Journal: Feed Review

Keyword:  地衣芽孢杆菌gyrB基因PCR;
Abstract: 根据地衣芽孢杆菌的gyrB基因序列设计一对引物,扩增片段大小为507 bp的基因片段,该方法对地衣芽孢杆菌DNA的扩增结果为阳性,对照菌株扩增结果均为阴性,对地衣芽孢杆菌检测的灵敏性为1 pg总DNA量,成功建立了地衣芽孢杆菌PCR检测方法,该方法具有快速、灵敏度高、特异性强等优点,为地衣芽孢杆菌的分离及鉴定奠定了良好的基础.
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