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Determination of shikimic acid in Pinus massoniana Lamb. from different producing areas and different species of pine needles by HPLC
Author(s): 
Pages: 52-55
Year: Issue:  1
Journal: Chinese Journal of Pharmaceutical Analysis

Keyword:  specific anti-flu medicineTamiflupine needleshikimic acidIllicium verumcomparison of different producing areas and speciesdrug resources development;
Abstract: Objective: To establish an HPLC method for determining shikimic acid contained in pine needles,so as to provide preliminary experimental data for quality control standards,and to compare the contents of Pinus massoniana Lamb. from different areas and different species of pine needles from Jilin province. Methods: HPLC and Ultimate XB-C18column( 4. 6 mm × 250 mm,5 μm) were adopted,with methanol-0. 5% phosphoric acid( 2 ∶ 98) as the mobile phase at the flow rate of 1. 0 m L·min-1. The column temperature was set at 25 ℃,and the UV detection wavelength was 213 nm. The optimum condition for determining shikimic acid in pine needles was decided by optimizing the parameters of preparation( decoloration,extraction time,solvent volume,immersion time and extraction frequency) and the contents of Pinus massoniana Lamb. from different areas and different species were determined. Results: The pretreatment of shikimic acid was ultrasonic extraction for 50 min with addition of 60 m L water. Shikimic acid showed a good linear relationship within the range of 0. 094-1. 875 μg( r = 0. 999 8),with the average recovery of 98. 8%( RSD = 0. 56%). Good precision and reproducibility was achieved with the RSDs smaller than 2. 0%. The content range was 2. 62%-0. 07% for different species of pine needles,and species with the relatively high contents were Pinus koraiensis Sieb. et Zucc.,and Pinus massoniana Lamb.,those with the lowest contents were Larix gmelinii( Rupr.) Rupr.. The content range was 4. 28%-2. 00% for Pinus massoniana Lamb.from different areas,and the highest and the lowest contents appeared in those from Guangxi and Henan. Conclusion: The method is simple,accurate,reproducible and can be used to determine the content of shikimic acid in pine needles. The content of shikimic acid in Pinus massoniana Lamb. is higher than that in other species. The content of shikimic acid is different among Pinus massoniana Lamb. from different areas and is higher in those from Guangxi,Sichuan,Zhejiang,and Jiangsu.
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