Bioactive constituents studies on Gentianopsis paludosa against UC fibrosis based on the qRT-RNA method
Author(s): LU Nianhua, ZHAO Huiqiao, LIU Xuefeng, JING Ming, CHEN Zhengjun
Pages: 628-631,662
Year: 2016 Issue: 11
Journal: Qilu Pharmaceutical Affairs
Keyword: Active component; Gentianopsis paludosa; Ulcerative colitis;
Abstract: Objective To study the bioactive constituents of Tibetan medicine Gentianopsis paludosa against UC fibro-sis.Methods Active fragment were obtained by silica gel and TLC from ethyl acetate active part which was extracted from Tibetan Gentianopsis paludosa.By method of qRT-PCR,collagen Ⅰ,collagen Ⅲ,α-SMA,and E-cad were detected to screen the active components after model rats being treated with active isolates.Results After purified and identified from ethyl acetate part using spectroscopy,four monomer compounds(1-hydroxy-3,7,8-Trimethoxyxanthones;1,8-Dihydroxy-3,7-Dimethoxyxanthones;1-hydroxy-3,7-Dimethoxyxanthones and 1,7-Dihydroxy-3,8-Dimethoxyxanthones)had sig-nificant bioactive on UC fibrosis.Conclusion The material basis of Gentianopsis paludosa against fibrosis concentrated in parts of ethyl acetate,and the active ingredient mainly owed to four xanthones.
Pages: 628-631,662
Year: 2016 Issue: 11
Journal: Qilu Pharmaceutical Affairs
Keyword: Active component; Gentianopsis paludosa; Ulcerative colitis;
Abstract: Objective To study the bioactive constituents of Tibetan medicine Gentianopsis paludosa against UC fibro-sis.Methods Active fragment were obtained by silica gel and TLC from ethyl acetate active part which was extracted from Tibetan Gentianopsis paludosa.By method of qRT-PCR,collagen Ⅰ,collagen Ⅲ,α-SMA,and E-cad were detected to screen the active components after model rats being treated with active isolates.Results After purified and identified from ethyl acetate part using spectroscopy,four monomer compounds(1-hydroxy-3,7,8-Trimethoxyxanthones;1,8-Dihydroxy-3,7-Dimethoxyxanthones;1-hydroxy-3,7-Dimethoxyxanthones and 1,7-Dihydroxy-3,8-Dimethoxyxanthones)had sig-nificant bioactive on UC fibrosis.Conclusion The material basis of Gentianopsis paludosa against fibrosis concentrated in parts of ethyl acetate,and the active ingredient mainly owed to four xanthones.
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