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Semiquantitative colorimetric detection of pesticide residues with enzyme substrate technique on paper-based chips
Author(s): 
Pages: 64-66
Year: Issue:  1
Journal: Occupational Health and Emergency Rescue

Keyword:  农药残留纸基芯片比色法;
Abstract: [目的]研究一种基于纸基芯片酶比色法的农药残留半定量快速检测卡其制作及检测方法.[方法]该速测卡由固定有乙酰胆碱酯酶的加样片、固定有碘化硫代乙酰胆碱的底物片、有淀粉-碘等间距条带的检测条及最外层的封闭固载膜组成.农药样品待测物通过加样孔接触加样片,依靠裸眼观测色带的退色数目即可确定被测物浓度.[结果]通过敌百虫标准农药样品系列测试,证明可通过条带退色数目半定量检测农药浓度,方法检出限为0.01mg/mL.[结论]这种快速检测卡操作简单迅速、结果直观、成本低廉,能做到半定量检测,可用于蔬菜、水果、土壤等样品中农药残留的现场检测.
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