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Effect of angiotensin Ⅱ on proliferation and activity of human endometrial epithelium cells
Author(s): 
Pages: 2474-2477
Year: Issue:  18
Journal: Occupation and Health

Keyword:  Angiotensin ⅡEndometrial epithelium cellsα-smooth muscle actinEpithelial cadherinCollagen type ⅠFibronectin;
Abstract: [Objective] To explore the effect of angiotensin Ⅱ (Ang Ⅱ) on proliferation and activity of human endometrial epithelium cells (EECs).[Methods] The EECs was isolated and cultured in vitro.There were the control group and Ang Ⅱ groups in the study.EECs in control group were cultivated by solution without serum,and EECs in Ang Ⅱ groups were cultivated by solution with different concentrations of Ang Ⅱ.The method of MTT was used to detect the proliferation of EECs.The contents of Collagen type Ⅰ (Col Ⅰ) and Fibronectin (FN) in the cultured supernatants were measured by enzyme-linked immunosorbent assay (ELISA).The expression levels of α-smooth muscle actin (α-SMA) and Epithelial cadherin (E-cadherin) in cytoplasm of EECs were detected by immunocytochemistry method.[Results] The proliferation rates of EECs in Ang Ⅱ groups significantly increased,and appeared a dose dependent trend.Compared with control group,α-SMA content (71.71±1.47) increased and E-cadherin content (25.32±1.43) decreased in AngⅡ groups,the contents of Col Ⅰ (0.774 7±0.048 0) and FN(0.758 0±0.042 0) both increased,and differences were statistically significant (P<0.05).[Conclusion] Ang Ⅱ can promote EECs proliferation,and stimulate EECs to change its vitality.
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