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chao gao xiao ye xiang se pu - san zhong si ji gan xian xing li zi zuo - zhi pu lian yong fa ding liang ce ding quan xue zhong sha shu mi
Author(s): 
Pages: 287-290
Year: Issue:  3
Journal: Occupational Health and Emergency Rescue

Keyword:  杀鼠醚超高效液相色谱-质谱/质谱联用仪全血定量检测;
Abstract: [目的]建立简便、灵敏、准确的全血中杀鼠醚的定量检测方法。[方法]采用超高效液相色谱-三重四级杆线性离子阱-质谱联用法建立全血中杀鼠醚的定量检测方法。样品经乙酸乙酯萃取,以乙酸铵(5 mmol/L)和甲醇为流动相进行梯度洗脱,在C18色谱柱上进行色谱分离,质谱检测采用电喷雾负离子化模式、多反应监测及增强子离子扫描模式。[结果]方法的检出限为3.4 ng/mL,工作曲线在10~1 200 ng/mL范围内线性良好,三个浓度水平的样品平均加标回收率为92.5%、94.3%和101.0%,血中杀鼠醚在4℃冰箱中至少可以保存7 d。[结论]建立的全血中杀鼠醚的定量检测方法,能减少杂质干扰,具有简便、灵敏、准确的优点,可以满足血中杀鼠醚的定量检测要求。
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