The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
Improvement in colonic inflammatory injury in rats via activating dorsal cholinergic neurons of vagus with electroacupuncture at sensitized acupoints
Author(s): 
Pages: 45-51
Year: Issue:  1
Journal: Chinese Acupuncture & Moxibustion

Keyword:  colitissensitized acupointsdorsal motor nucleus of vagusCh AT+ neuronsanti-inflammation;
Abstract: Objective To observe the effect of electroacupuncture(EA) at sensitized acupoints on choline acetyltransferase positive(Ch AT+) neurons in dorsal motor nucleus(DMV) of brainstem vagus in the colitis model rats and explore the mechanism of the improvement in colonic inflammatory injury in the rats. Methods A total of 79 male SD rats were randomized into five groups, i.e. a normal group(20 rats), a normal plus sensitized acupoint group(5 rats), a model group(34 rats), an EA-1 group(15 rats) and an EA-2 group(5 rats). In the model group and the EA groups, 5% dextran sulfate sodium(DSS) was adopted for 6-day free drinking to establish colitis model rats. By injecting Evans blue(EB) into the caudal vein in the model rats, the sensitized acupoints were determined. Afterwards, in the normal plus sensitized acupoint group, the EA-1 group and the EA-2 group, EA was exerted at the sensitized acupoints, with disperse-dense wave, 2 Hz/15 Hz in frequency and 2 m A in intensity, for 30 min of intervention each day. The intervention lasted for 6 days in the EA-1 group and for 1 day in both the normal plus sensitized acupoint group and the EA-2 group. On day 0, 7 and 13 of experiment, successively, the score of disease activity index(DAI), the score of colonic histological damage, as well as the changes in the mechanical paw withdrawal threshold and thermal paw withdrawal latency were evaluated in the normal group, the model group and the EA-1 group. On day 7 of experiment, using immunofluorescence staining, the activation of different lamina neurons of spinal dorsal horn and Ch AT+ neurons in DMV was observed in the normal group, the normal plus sensitized acupoint group, the model group and the EA-2 group separately. Results The EB extravasating areas were distributed in the segments from T12 to S1 on the body surface of colitis model rats, mainly focusing at L2 and L5. Therefore, "Shangjuxu"(ST 37) was taken as the sensitized acupoint. Compared with the normal group on day 7 and 13 of experiment, the mechanical paw withdrawal threshold were reduced(P<0.001), DAI scores and the scores of colonic histological damage were increased(P<0.001) in the model group. Compared with the normal group on day 7 of experiment, thermal paw withdrawal latency in the model group was reduced(P<0.001). Compared with the model group on day 13 of experiment, the mechanical paw withdrawal threshold was increased(P<0.001), DAI score and the score of colonic histological damage were reduced(P<0.01, P<0.05) in the EA-1 group. Compared with the normal group, the activated numbers of the neurons in superficial laminae(Ⅰand Ⅱ) at spinal dorsal horn of L4 to L6 and Ch AT+ neurons in DMV were increased in the normal plus sensitized acupoint group and the model group(P<0.05, P<0.01). Compared with the normal plus sensitized acupoint group and the model group, the activated numbers of the neurons in superficial laminae at spinal dorsal horn of L4 to L6 and Ch AT+ neurons in DMV were increased in the EA-2 group(P<0.001). Conclusion The segmental dominance(acupoints) from T12 to S1 on the body surface of colitis rats is sensitized. EA at sensitized acupoints effectively relieves colonic inflammatory injury, which is probably by activating superficial lamina neurons of spinal dorsal horn and Ch AT+ neurons of DMV. +
Related Articles
No related articles found