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Study on the Conditions of Extracting and Purifying of Polysaccharide from Marine Microalgae
Author(s): 
Pages: 9-12
Year: Issue:  1
Journal: OCEAN TECHNOLOGY

Keyword:  海洋微藻多糖提取纯化;
Abstract: 研究了提取介质、超声条件和洗脱条件对海洋微藻多糖提取纯化效果的影响.实验结果表明不同pH的提取介质可以提取得到不同种类的多糖,超声条件以300 W、20 min最佳,用葡聚糖凝胶Sephadex-G200柱(长50 cm,直径2 cm)纯化粗多糖,当加样量为4 ml,用0.2 mol/L NaCl溶液洗脱效果最好.
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